Equipment
Lattice Lightsheet 7 (under demonstration for evaluation from November, 2022)
Long-term volumetric imaging of live cells at subcellular resolution with minimum photodamage
Use of #1.5 (~0.17 mm thickness) glass bottom dishes and glass bottom multi-well plates is allowed
3 laser lines (488, 561, 640)
3 types of lightsheet with length [um] x thickness [nm]: 15 x 550, 30 x 700, 100 x 1,400
Near-isotropic resolution: 330 nm (xy) and 500-1000 nm (z)/290 nm (xy) and 450-900 nm (z) with deconvolution
Field of View: x: defined by scan range, y: 300 um, z: typically 50-100 um, up to 200 um. Tiling in the y direction is available for large sample.
Simultaneous dual color imaging by 2 sCMOS cameras
Elyra 7 with Lattice SIM^2 (started service in February, 2022)
Light efficient 4D super-resolution live-cell imaging
4 laser lines (405, 488, 561, 642)
Fast (up to 255 fps with 2D Burst mode) and gentle super-resolution imaging by lattice patterned illumination
which is more light efficient and gives better contrast compared with classic SIM by stripe patterned illumination
Lattice SIM^2 mode with an advanced deconvolution method provides 60-nm (xy) and 200-nm (z) resolutions
SIM^2 Apotome mode for fast optical sectioning with nealy isopropic 140-nm (xy) and 275-nm (z) resolutions for 40x objective lens
Simultaneous dual color imaging by 2 sCMOS cameras
LSM 980 with Airyscan 2 (since 2019)
Long-term live-cell & 3D confocal/super-resolution/2-photon imaging
7 laser lines (405, 445, 488, 514, 561, 639, Chameleon Ultra II 680-1080)
Airyscan 2 super-resolution imaging with 120-nm (xy) and 350-nm (z) resolutions, further improved to 90-nm (xy) and 270-nm (z) resolutions by joint deconvolution (jDCV)
Airyscan 2 fast and gentle multiplex mode with 4x and 8x acquisition speeds
Spectral unmixing by 32ch GaAsP detector array
Improved operation and analysis interface by Zen Blue, Sample navigator, and Zen Connect
LSM 880 with Airyscan (since 2016)
Long-term live-cell & 3D confocal/super-resolution imaging
6 laser lines (405, 458, 488, 514, 561, 633)
Airyscan super-resolution imaging with 120-nm (xy) and 350-nm (z)
resolutions
Airyscan fast mode with 4x acquisition speed
Spectral unmixing by 32ch GaAsP detector array
Improved system stability compared with LSM780 and earlier
LSM 780 (since 2010)
Long-term live-cell & 3D confocal imaging
7 laser lines (405, 440, 458, 488, 514, 561, 633)
Spectral unmixing 32ch GaAsP detector array
Molecular interaction detection by FCS/FCCS (ConfoCor3 with APD
detector)
LSM 710 (since 2014)
Long-term live-cell & 3D confocal imaging: all microscopes in the
Innovation Core are equipped with autofocus unit and stage-top incubator
5 laser lines (458, 488, 514, 561, 633)
2ch GaAsP detectors (BiG)
Tiling/stitching for multiple fields