[January 24, 2024] New publication: Fukute et al., Commun Biol
[December 1, 2023] New publication: Ando et al., Nat Methods
[October 2, 2023] CRA (Cooperative Research Agreement) on the use of StayGold, a highly photostable and bright green fluorescent protein, at the Analysis Center
[August 1, 2023] New publication: Yu et al., Nat Commun
[June 7, 2023] New publication: Fujiwara et al., J Cell Biol
[June 2-5, 2023] Lattice Lightsheet 7 Demonstration Sessions
Date & Time
June 2 (Fri) - 5 (Mon), 1 - 2 sessions per day, including long-term observations
Place
ZEISS-iCeMS Innovation Core, Room 305 of Research Bldg. No.1 Annex (#33)
[April 18 and 19, 2023] Demonstration Seminar on arivis Pro
On April 18 afternoon and April 19, technical support and discussion sessions for reserchers from iCeMS and other departments of Kyoto University were arranged.
[February 6-8, 2023] Lattice Lightsheet 7 Demonstration Sessions
Date & Time
February 6 (Mon) - 8 (Wed), 1 - 2 sessions per day, 3 hrs each
Place
ZEISS-iCeMS Innovation Core, Room 305 of Research Bldg. No.1 Annex (#33)
[January 19-20, 2023] Lattice Lightsheet 7 Demonstration Sessions
Date & Time
January 19 (Thu) and 20 (Fri), 2 sessions per day, 3 hrs each
Place
ZEISS-iCeMS Innovation Core, Room 305 of Research Bldg. No.1 Annex (#33)
[January 5, 2023] New publication: Ogasawara and Ueda, J Chem Biol
[December 13-16, 2022] Lattice Lightsheet 7 Workshop
Lattice Lightsheet 7 provides lattice lightsheet fluorescence microscopy for live cell imaging with minimum photodamage while allowing your use of standard glass bottom dishes/well plates. With the automated system calibration and easy-to-use interface, volumetric imaging of cellular events ranging from dynamics of subcellular structures to multicellular interactions over hours and days becomes available to everyone.
Dr. Kirstin Elgass (Business Sector Life Sciences, ZEISS Research Microscopy Solutions, Jena, Germany) gave an online seminar on Lattice Lightsheet technology and its applications to various live-cell imaging on December 13, 16:00-17:00. On the next three days, onsite demonstration sessions using own sample (about 2 hrs each) were arranged.
Date & Time
Technical seminar:
December 13 (Tue), 2022, 16:00-17:00
Demonstration:
December 14 (Wed) – 16 (Fri), 3 sessions per day, 2 hrs each
Place
Technical seminar: Online via Zoom
Demonstration: ZEISS-iCeMS Innovation Core, Room 305 of Research Bldg. No.1 Annex (#33)
Language
English (seminar); Japanese or English (demonstration)
Flyer
You will have access to the system installed at ZEISS-iCeMS Innovation Core for several months. Please contact ZEISS-iCeMS Innovation Core if you get interested in long-term use of the system.
[October 31-November 4, 2022] "Applicathon" Workshop at Kyoto University
[October 31, 2022] Lattice Lightsheet 7 is under demonstration for evaluation.
[July 4 and 5, 2022] Meeting and discussion at Kyoto University
Dr. Xianke Shi gave a technical seminar entitled "Recent Advances in ZEISS Microscopy for Fluorescence Live Cell Imaging". In addition, 11 research groups from iCeMS and other departments of Kyoto University presented their research and discussed current problems and future technical requirements.
Schedule
Meeting by the Innovation Core member:
July 4 (Mon), 2022, 11:00-13:00
Technical seminar:
July 4 (Mon), 2022, 13:30-14:15
Dr. Xianke Shi (ZEISS Microscopy Life Sciences, APAC)
Recent Advances in ZEISS Microscopy for Fluorescence Live Cell Imaging
Disucussion with the researchers:
July 4 (Mon) and July 5 (Tue)
[April 26, 2022] New publication on StayGold, super-stable fluorescent protein: Hirano et al., Nat Biotechnol
[March 28, 2022] Online Seminar (via Zoom): Towards fast and gentle super-resolution microscopy
In this online seminar, first, the scientific lecture on the development of novel fluorescent proteins to improve observations of various cellular processes is given by Dr. Atsushi Miyawaki (RIKEN CBS). Next, the technical lecture on the features of two ZEISS super-resolution microscopes “Elyra 7 with Lattice SIM2” and “LSM 980 with Airyscan 2”, available at ZEISS-iCeMS Innovation Core, is given by Dr. Klaus Weisshart (ZEISS Jena, Germany).
Elyra 7 was just installed as shared equipment of MaCBES (Main Campus Base of Equipment Support), Kyoto University in December 2021. After the seminar, we will have onsite demonstration sessions for Elyra 7 using your samples (about 2 hrs each) on March 29, 30, and 31.
Please register for the online seminar and the onsite demonstration session by March 18 from the Registration URL below. Zoom meeting information will be announced by e-mail.
Date: March 28 (Mon), 2022
14:00-14:45 Atsushi Miyawaki (Riken CBS)
Continuity in Space and Time
14:45-15:45 Klaus Weisshart (ZEISS Jena, Germany)
Latest developments in confocal and widefield based super-resolution microscopy using the example of Carl Zeiss Airyscan 2 and ELYRA 7
Language: English
Registration: https://forms.gle/Xtr4skoksw4zSpK67 (closed)
Flyer
[December 2, 2021] Elyra 7 with Lattice SIM^2 was installed.
[September 28, 2021] New publication: Minakawa et al., J Extracell Vesicles
[June 1, 2021] Publication list updated.
[April 7, 2020] iCeMS Specially Appointed Professor Fumiyoshi Ishidate, member of ZEISS-iCeMS Innovation Core, was selected to receive the "Outstanding Support for Research Award" of the "Commendation for Science and Technology by the Minister of Education, Culture, Sports, Science and Technology" for 2020.
Prof. Ishidate has made great contributions to iCeMS imaging facility for these 10 years, especially as a mentor of live cell imaging using ZEISS microscopes. He provided one-on-one training sessions to a cumulative total of 600 researchers inside and outside the university. In addition, through the collaboration with iCeMS Kageyama group, he developed the "long-term microscopic observation technique for weak signals in living cells" taking advantage of his deep knowledge of live cell samples and skills to operate advanced confocal microscopes. Using the developed technique, time-lapsed imaging of weak signals from the fate-determination transcription factors in neural progenitor cells was successfully performed for the first time. It resulted in the finding that the multipotent state correlates with oscillatory expression of several fate-determination factors, whereas the differentiated state correlates with sustained expression of a single factor (Imayoshi et al., 2013, 2015), which attracted worldwide attention.
Prof. Ishidate also made continued efforts to establish the long-standing partnership between iCeMS and ZEISS German headquarters, and based on the partnership, we were able to launch the ZEISS-iCeMS Innovation Core in 2019.
Congratulations, Ishidate-san!
References
1. Imayoshi I, Isomura A, Harima Y, Kawaguchi K, Kori H, Miyachi H, Fujiwara T, Ishidate F, Kageyama R. Oscillatory control of factors determining multipotency and fate in mouse neural progenitors. Science 342: 1203-1208 (2013).
2. Imayoshi I, Ishidate F, Kageyama R. Real-time imaging of bHLH transcription factors reveals their dynamic control in the multipotency and fate choice of neural stem cells. Front Cell Neurosci 9: 288 (2015).
[January 27-29, 2020] ZEISS Elyra 7 with Lattice SIM Workshop
In Lattice SIM, the sample area is illuminated with a lattice pattern instead of grid lines. The lattice pattern gives higher contrast and 2x higher sampling efficiency compared with conventional SIM, so that you need less illumination. This is a breakthrough in light efficiency, enabling fast live imaging of 3D volumes at excellent resolutions (120 nm in XY and 300 nm in Z) while minimizing photodamage.
Dr. Bernhard Zimmermann (Senior Director Business Sector Life Sciences, ZEISS Research Microscopy Solutions, Jena, Germany) gave a seminar on Lattice SIM technology and its comparison to other super-resolution methods. After the seminar, demonstration sessions using own samples (about 2 hrs each) were arranged.
Date & Time
Technical seminar:
January 27 (Mon), 2020, 13:30-14:30
Demonstration:
January 27 (Mon), 2020, 1 session after the seminar
January 28 (Tue), 2020, 3 sessions
January 29 (Wed), 2020, 3 sessions
Place
Technical seminar: Room 119 of Research Bldg. No.1 (#32) at Main Campus of Kyoto University
Demonstration: ZEISS-iCeMS Innovation Core, Room 305 of Research Bldg. No.1 Annex (#33)
Language
English
Flyer
You will have access to the system installed at ZEISS-iCeMS Innovation Core for several months. Please contact ZEISS-iCeMS Innovation Core if you get interested in long-term use of the system.
[December 23 and 24, 2019] ZEISS LSM 980 with Airyscan2 Briefing Session
The microscope provides the capability of fast and gentle 4D super-resolution imaging over large view fields. The difference between Airyscan2 (confocal-based super-resolution) and ZEISS Elyra 7 with Lattice SIM (newly developed lattice structured illumination-based super-resolution suitable for live imaging) was also discussed.
Masako Yamaguchi (ZEISS Research Microscopy Solutions) gave technical seminar and quick demonstration at the microscope system.
Date & Time
Slot 1: Monday, December 23, 2019 13:00-14:00 (Japanese)
Slot 2: Monday, December 23, 2019 15:00-16:00 (Japanese)
Slot 3: Tuesday, December 24, 2019 13:00-14:00 (Japanese)
Slot 4: Tuesday, December 24, 2019 15:00-16:00 (English)
Place
Technical seminar: Room 119 of Research Bldg. No.1 (#32) at Main Campus of Kyoto University
Demonstration: ZEISS-iCeMS Innovation Core, Room 304 of Research Bldg. No.1 Annex (#33)
Flyer
[October 28, 2019] ZEISS-iCeMS Innovation Core Founding Commemorative Symposium & Opening Ceremony
Ko-ichiro Tanaka
(Kyoto Univ Dept of Physics / iCeMS)
Terahertz Imaging and Nearfield Microscopy
Naoki Watanabe
(Kyoto Univ Dept of Medicine)
Single-Molecule Dissection of Physico-Biochemical Coupling in Actin System
Ryoichiro Kageyama
(Kyoto Univ Inst for Frontier Life & Med Sci / iCeMS)
Live Imaging of Dynamic Expression of the Mouse Segmentation Clock Gene Hes7
Date & Time
October 28, 2019
13:00-15:00
Venue
KUIAS/iCeMS Main Building 2F Seminar Room (A207)
Flyer
After the Symposium, Opening Ceremony of ZEISS-iCeMS Innovation Core and Signing of the Collaborative Research Agreement between ZEISS and KUIAS/iCeMS Kyoto University were holded.
Link to Press Release by ZEISS Research Microscopy Solutions
Link to Kyoto University News
Link to iCeMS News (Japanese)